OPEN ACCESS
PEER-REVIEWED |
RESEARCH ARTICLE
Efektivitas pemberian hormon pertumbuhan rekombinan ikan kerapu kertang (Epinephelus lanceolatus, Bloch 1790) melalui perendaman dan oral terhadap pertumbuhan elver ikan sidat (Anguilla bicolor bicolor)
Article Sidebar
How to Cite
The article can trace at:
Main Article Content
Abstract
Indonesian eel (Anguilla bicolor bicolor) has recently been cultivated intensively in Indonesia. The growth of Indonesian eel is slow. This study was performed to determine a proper delivery method of recombinant giant grouper (Epi-nephelus lanceolatus) growth hormone (rElGH) and observe aquaculture and nutrient related performances of Indonesian eel elver after rElGH treatment. Three delivery methods of rElGH, namely immersion, orally, and combination of immersion and orally were tested. The dosage of rElGH by immersion (12 mg L"1 water) and orally administration (30 mg kg-1 feed) were obtained in previous studies. Fish were maintained in 23 L glass aquaria for 120 days, at density of 45 fish in each aquarium. The results showed that higher (P<0.05) biomass of harvest (73.68±2.07 g) was obtained in immersion and oral combination treatment, by increment of about 102.9% compared to control (36.32±0.97 g). Administration of rElGH also improved protein and lipid retentions by 142.5%, and 720.0% compared to those of control, respectively (P<0.05). In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (4.75) was 55.15% lower (P<0.05) compared to that of control (7.37). Combination of rElGH immersion and oral administrations also increased hepatosomatic index, and insuline-like growth factor-1 gene expression level. Thus, rElGH administration via immersion and oral combination was a proper method to improve growth of eel elver, and application of rElGH can be useful to increase aquaculture production.
Abstrak
Ikan sidat (Anguilla bicolor bicolor) telah dibudidayakan secara intensif di Indonesia. Pertumbuhan ikan sidat relatif lambat. Penelitian ini dilakukan untuk menentukan metode pemberian hormon pertumbuhan rekombinan ikan kerapu kertang (Epinephelus lanceolatus) (rElGH) untuk memacu pertumbuhan, dan mengevaluasi performa budi daya dan pemanfaatan pakan pada elver ikan sidat. Tiga metode pemberian rElGH diuji, yakni perendaman, oral, dan kombinasi perendaman dan oral. Dosis rElGH yang digunakan merupakan hasil penelitian sebelumnya, yakni 12 mg L-1 untuk me-tode perendaman dan 30 mg kg-1 pakan untuk metode oral. Ikan dipelihara dalam akuarium volume 23 L selama 120 hari, dengan padat tebar 45 ekor per akuarium. Hasil penelitian menunjukkan bahwa biomassa tertinggi (p<0,05) diper-oleh pada perlakuan kombinasi perendaman dan oral (73,68±2,07 g), sekitar 102,9% lebih tinggi daripada kontrol tanpa pemberian rElGH (36,32±0,97 g). Perlakuan rElGH juga meningkatkan retensi protein dan lemak, masing-masing 142,5% dan 720,0% lebih tinggi daripada kontrol (p<0,05). Selanjutnya, perlakuan rElGH meningkatkan nafsu makan, dan konversi pakan (4,75) sekitar 55,2% lebih rendah (p<0,05) daripada kontrol (7,37). Perlakuan kombinasi perendaman dan oral juga meningkatkan indeks hepatosomatik, dan ekspresi gen insuline-like growth factor I. Sebagai kesimpul-an, pemberian rElGH melalui kombinasi perendaman dan oral merupakan metode terbaik dalam memacu pertumbuhan elver ikan sidat, dan penerapan rElGH dapat digunakan untuk meningkatan produksi budi daya.
Downloads article
Article Details
Copyright (c) 2014 Jurnal Iktiologi Indonesia |
|
|
References
Acosta JR, Morales R, Morales M, Alonso M, Estrada MP. 2007. Pichia pastoris expressing recombinant tilapia growth hormone accelerates the growth of tilapia. Biotechnology Letters, 29(11): 1671-1676.
Alimuddin, Lesmana I, Sudrajat AO, Carman O, Faizal I. 2010. Production and bioactivity potential of three recombinant growth hormones of farmed fish. Indonesian Aquaculture Journal, 5(1): 11-16.
Alimuddin. 2003. Introduction and expression of foreign A6-desaturase-like gene in a teleostean fish. Master Thesis. Tokyo University of Fisheries. 41 p.
Cook JT, McNiven MA, Richardson GF, Sutter-lin AM. 2000. Growth rate, body composition and feed digestibility/conversion of growth enhanced Atlantic salmon (Salmo salar). Aquaculture, 188(1-2): 15-32.
Debnanth S. 2010. A review on the physiology of insulin-like growth factor-I (IGF-I) peptide in bony fishes and its phylogenetic correlation in 30 different taxa of 14 families of teleosts. Advances in Environmental Biology, 5(1):31-52.
Devlin RH, Biagi CA, Yesaki TY, 2004. Growth, viability and genetic characteristics of GH transgenic coho salmon strains. Aquaculture, 236(1-4):607-632.
Duan C, Hirano T. 1992. Effects of insulin-like growth factor-I and insulin on the in vitro uptake of sulfate by eel branchial cartilage: evidence for the presence of independent hepatic and pancreatic sulfation factors. Journal of Endocrinology, 133(2):211-219.
Duan C. 1998. Nutritional and developmental regulation of insulin-like growth factors in fish. The Journal of Nutrition, 128(2):306S-314S.
Duguay SJ, Lai-Zhang J, Steiner DF, Funkens-tein B, Chan SJ. 1996. Developmental and growth in juvenile Catla catla. Indian Journal of Fisheries, 48(1):27-33.
Duguay SJ, Swanson P, Dickhoff WW. 1994. Differential expression and hormonal regulation of alternatively spliced IGF-I mRNA transcript in salmon. Journal of Molecular Endocrinology, 12(1):25-37.
Farmanfarmaian A, Sun LZ. 1999. Growth hormone effects on essential amino acid absorption, muscle amino acid profile, N retention and nutritional requirements of striped bass hybrids. Genetic Analysis, 15(3-5):107-113.
Fauconneau B, Mady MP, Le Bail PY. 1996. Effect of growth hormone on muscle protein synthesis in rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). Fish Physiology and Biochemistry, 15(1): 49-56.
Fu C, Cui Y, Hung SSO, Zhu Z. 1998. Growth and feed utilization by F4 human growth hormone transgenic carp fed diets with different protein levels. Journal of Fish Biology, 53(1):115-129.
Haghighi M, Sharif RM, Sharifpour I, Sepahdari A, Lashtoo AGR. 2011. Oral recombinant bovine somatotropin improves growth performance in rainbow trout (Oncorhynchus mykiss). Iranian Journal of Fisheries Sciences, 10(3):415-424.
Handoyo B, Alimuddin, Utomo NBP. 2012. Growth, feed conversion and retention, and proximate of eel juvenile treated by immersion of recombinant giant grouper growth hormone. Jurnal Akuakultur Indonesia, 11(2):132-140.
Handoyo B. 2013. Respons benih ikan sidat terhadap hormon pertumbuhan rekombinan ikan kerapu kertang melalui perendaman dan oral. Tesis. Institut Pertanian Bogor. 53 hlm.
Hardiantho D, Alimuddin, Prasetiyo AE, Yanti DH, Sumantadinata K. 2012. Performa benih ikan nila diberi pakan mengandung hormon pertumbuhan rekombinan ikan mas dengan dosis berbeda. Jurnal Akuakultur Indonesia, 11(1): 17-22.
Hashimoto H, Mikawa S, Takayama E, Yoko-yama Y, Toyohara H, Sakaguchi M. 1997. Molecular cloning and growth ormoneregulated gene expression of carp insulin-like growth factor-I. Biochemistry and Molecular Biology International, 41(5):877-886.
Haukenes AH, Barton BA, Bollig H. 2008. Cortisol responses of pallid sturgeon and yellow perch following challenge with lipopolysaccharide. Journal of Fish Biology, 72(3):780-784.
Heinsbroek LTN, Van Hoff PLA, Winkels W, Tank MWT, Schrama JW, Verreth JAJ. 2007. Effects of feed composition on life history developments in feed intake, metabolism, growth and body composition of European eel (Anguilla anguilla). Aquaculture, 267(1-4):175-187.
Irmawati, Alimuddin, Zairin MJr, Suprayudi MA, Wahyudi AT. 2012. Peningkatan laju pertumbuhan benih ikan gurami (Osphronemus goramy Lac.) yang direndam dalam air yang mengandung hormon pertumbuhan ikan mas. Jurnal Iktiologi Indonesia, 12(1): 13-23.
Kopchick JJ, Andry JM. 2000. Growth hormone (GH), GH receptor, and signal transduction. Molecular Genetics and Metabolism, 71(1-2):293-314.
Leedom TA, Uchida K, Yada T, Richman NH, Byatt JC, Collier RJ, Hirano T, Grau EG. 2002. Recombinant bovine growth hormone treatment of tilapia: growth response, metabolic clearance, receptor binding and immunoglobulin production. Aquaculture, 207(3-4):359-380.
McCormick SD. 2001. Endocrine control of osmoregulation in the teleost fish. American Zoologist, 41(4):781-794.
Moriyama S, Hiroshi Y, Seiji S, Toshio A, Tet-suya H, Hiroshi K. 1993. Oral administration of recombinant salmon growth hormone to rainbow trout (Oncorhynchus mykiss). Aquaculture, 112(1):99-106.
Moriyama S, Ayson FG, Kawauchi H. 2000. Growth regulation by insulin-like growth factor I in fish. Bioscience, Biotechnology, and Biochemistry, 64(8):1553-1562.
Moriyama S, Kawauchi H. 1990. Growth stimulation of juvenile salmonids by immersion in recombinant salmon growth hormone. Nippon Suisan Gakkaishi, 56(1):31-34.
Nam YK, Noh JK, Cho YS, Cho HJ, Cho KN, Kim CG, Kim DS. 2004. Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach (Misgurnus mizolepis). Transgenic Research, 10(4):
-362.
Nayak PK, Misra J, Mishra TK, Pandey AK, Singh BN, Ayyappan S. 2001. Evaluation of the potential for using hGH to enhance growth in juvenile Catla catla. Indian Journal of Fisheries, 48(1):27-33.
O'Connor PK, Reich B, Sheridan MA. 1993. Growth hormone stimulates hepatic lipid mobilization in rainbow trout (Oncorhynchus mykiss). Journal of Comparative Physiology, 163(5):427-431.
Oommen OV, Johnson B. 1998. Metabolic effects of ovine growth hormone in a teleost, Anabas testudineus. Annuals of the New York Academy of Sciences, 839(1):380-381.
Promdonkoy B, Warit S, Panyim S. 2004. Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli. Biotechnology Letters, 26(8):649-653.
Rahman MA, Mak R, Ayad H, Smith A, Maclean N. 1998. Expression of a novel piscine growth hormone gene results in growth enhancement in transgenic tilapia (Oreochromis niloticus). Transgenic Research, 7(5): 357-369.
Sakai M, Kajita Y, Kobayashi M, Kawauchi H. 1997. Immunostimulating effect of growth hormone: in vivo administration of growth hormone in rainbow trout enhances resistance to Vibrio anguillarum infection. Veterinary Immunology and Immunopathology, 57(1-2):1-6.
Santiesteban D, Martin L, Arenal A, Franco R, Sotolongo J. 2010. Tilapia growth hormone binds to a receptor in brush border membrane vesicles from the hepatopancreas of shrimp (Litopenaeus vannamei). Aquaculture, 306(1-4):338-342.
Shamblott MJ, Cheng CM, Bolt D, Chen TT. 1995. Appearance of insulin-like growth factor mRNA in the liver and pyloric caeca of a teleost in response to exogenous growth hormone. Proceedings of the National Academy of Sciences of the United States of America, 92(15):6943-6946.
Silverstein JT, Wolters WR, Shimizu M, Dickh-off WW. 2000. Bovine growth hormone treatment of channel catfish: strain and temperature effects on growth, plasma IGF-I levels, feed intake and efficiency, and body composition. Aquaculture, 190(1-2):77-88.
Subaidah S, Carman O, Sumantadinata K, Sukenda, Alimuddin. 2012. Respons pertumbuhan dan ekspresi gen udang vaname (Litopenaeus vannamei) setelah direndam dalam larutan hormon pertumbuhan rekombinan ikan kerapu kertang. Jurnal Riset Akuakultur, 7(3):337-352.
Subaidah S. 2013. Respons pertumbuhan, imunitas dan ekspresi gen udang vaname (Litopenaeus vannamei) yang diberi hormon pertumbuhan rekombinan. Disertasi. Institut Pertanian Bogor. 114 hlm.
Syazili A, Irmawati, Alimuddin, Sumantadinata K. 2012. Pertumbuhan dan kelangsungan hidup juvenil ikan gurami yang direndam dalam hormon pertumbuhan rekombinan dengan frekuensi berbeda. Jurnal Akuakultur Indonesia, 11(1):23-27.
Syazili A. 2012. Aplikasi hormon pertumbuhan rekombinan melalui perendaman untuk memacu pertumbuhan benih ikan gurami (Osphronemus goramy). Tesis. Institut Pertanian Bogor. 52 hlm.
Tomiyama T, Hibiya T. 1977. Fisheries in Japan (Eel). Japan Marine Product Photo Materials Association. 225 p.
Tsai HJ, Hsih MH, Kuo JC. 1997. Escherichia coli produced fish growth hormone as a feed additive to enhance the growth of juvenile black seabream (Acanthopagrus schlegeli). Journal of Applied Ichthyology, 13(2):78-82.
Volkoff H, Canosa LF, Unniappan S, Cerda-Reverterfer JM, Bernier NJ, Kelly SP, Peter RE. 2005. Neuropeptides and the control of food intake in fish. General and Comparative Endocrinology, 142(1-2):3-19.
Vong QP, Chan KM, Cheng CH. 2003. Quantification of common carp (Cyprinus carpio) IGF-I and IGF-II mRNA by realtime PCR: differential regulation of expression by GH. Journal of Endocrinology, 178(3):513-521.
Walker RL, Buret AG, Jackson CL, Scott KG, Bajwa R, Habibi HR. 2004. Effects of growth hormone on leucine absorption, intestinal morphology, and ultrastructure of the goldfish intestine. Canadian Journal of Physiology Pharmacology, 82(11):951-959.
Watanabe T. 1998. Fish nutrition and mariculture. Department of Aquatic Bioscience. Tokyo University of Fisheries. JICA. 233 p.
Yandes, Affandi R, Mokoginta I. 2003. Pengaruh pemberian selulosa dalam pakan terhadap kondisi biologis benih ikan gurami (Osphronemus gourami Lac). Jurnal Iktiologi Indonesia, 3(1):27-33.
Zhu T, Goh ELK, Graichen R, Ling L, Lobie PE. 2001. Signal transduction via the growth hormone receptor. Cellular Signalling, 3(9):599-616.